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- CAS号：
24303-61-5
2,2,3,3-Tetramethylcyclopropanecarbonyl Chloride (>90%)
24303-61-5
C₈H₁₃ClO
160.64
Off-White to Pale Pink Semi-Solid to Solid
Benzene (Sparingly), Chloroform
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文献和实验2,2,3,3-Tetramethylcyclopropanecarbonyl Chloride (>90%)
24303-61-5
C₈H₁₃ClO
160.64
Off-White to Pale Pink Semi-Solid to Solid
Benzene (Sparingly), Chloroform
Competent cells, calcium chloride method, E. coli, description
1/100 - 1/200 to 25, 50 or 100 ml 2 X YT broth in flasks 5 - 10 X culture volume (i.e. 25 ml in 250 ml flask etc.). Grow to early log phase (OD600 = 0.2 - 0.4) (90 - 180 min depending on the strain) Collect cells by centrifugation
Plasmid DNA Isolation, Alkaline Lysis（碱裂解法分离质粒DNA）
volume to 500ml with water. Soln 3 (B): per 100 mls:29.4 g potassium acetate/5 ml 90% formic acid/add water to bring to final volume. Filter if turbid. Protocol: Culture Volume 30-35 ml 1.5 ml 500 ml 1. Centrifuge to pellet and add soln 1 on ice for 15-30 min
59.978 p (g/ml) 20o C 1.0765 1.1531 1.2286 1.3032 1.3773 1.4141 Cl- CaCl2 Calcium Chloride, CaCl2 Mw=110.98g/M; CaCl2 x2H2 O Mw=147.02g/M; CaCl2 x 6H2 O Mw=219.08g/M; (store at NT
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