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- CAS号：
2231676-00-7
6-Methyl-3,6-diazabicyclo[3.1.1]heptane Dihydrochloride
2231676-00-7
C₆H₁₂N₂ • 2HCl
N/A
White to Off-White Solid
DMSO (Slightly, Sonicated), Methanol (Slightly), Water (Slightly)
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文献和实验6-Methyl-3,6-diazabicyclo[3.1.1]heptane Dihydrochloride
2231676-00-7
C₆H₁₂N₂ • 2HCl
N/A
White to Off-White Solid
DMSO (Slightly, Sonicated), Methanol (Slightly), Water (Slightly)
Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS
on a Millipore device with a nitex filter; rinse well with 0.1% Triton X-100 from a squirt bottle. Transfer embryos to 3:1 heptane:fix with a paint brush and fix for 20-40 min. Fix in a glass scintillation vial using 6.1ml HEPES Fix Mix + 0.75ml 37
Single and Double FISH protocols for Drosophila
1. Chlorine bleach; diluted 1:1 with water. 2. 40% formaldehyde solution (prepared fresh from paraformaldehyde as described below). 3. 10X PBS solution. 4. Heptane. 5. Methanol. 6. 20 ml Disposable Glass Scintillation Vials (Fisher).
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