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13-O-Ethylamphotericin B (>80%)
N/A
C₄₉H₇₇NO₁₇
952.13
Yellow to Orange Solid
DMSO (Slightly), Methanol (Slightly)
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文献和实验13-O-Ethylamphotericin B (>80%)
N/A
C₄₉H₇₇NO₁₇
952.13
Yellow to Orange Solid
DMSO (Slightly), Methanol (Slightly)
Mouse B cell isolation with magnetic beads
and incubate on ice for 15min; l Negative : 1.Resuspend cells with MACS to 400 ul and 80 ul B cell Biotin each spleen,incubate on ice for 15 min; 2. Add 300 MACS and 200 anti-biotin Beads each spleen, incubate on ice for 15 min.
A/B/C Random Amplification Protocol
Material DNA fragmentation Part A: Random priming Part B: Dye-primer PCR Hybridization reagents Sau3a (NEB) Sau3a 10x buffer BSA 10 mg/mL Sequenase, T7 DNA pol, 13 U/µ
b-Galactosidase Activity Assay
). Chloroform is easier to pippete if the air in the pippete tip is saturated by drawing up and releasing chloroform several times.Vortex; equilibrate the tubes 5' in a 28 C water bath.AssayStart reaction by adding 0.2 mL substrate, o -nitrophenyl-b -D
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