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- CAS号：
609-13-2
Ethyl 2-Bromo-3-oxobutanoate (>80%)
609-13-2
C₆H₉O₃Br
209.03
Colourless Oil
Chloroform (Slightly), Ethyl Acetate (Slightly), Methanol (Slightly)
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文献和实验Ethyl 2-Bromo-3-oxobutanoate (>80%)
609-13-2
C₆H₉O₃Br
209.03
Colourless Oil
Chloroform (Slightly), Ethyl Acetate (Slightly), Methanol (Slightly)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dense products have been devised. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium
Phycoerythrin conjugation protocol
reagents and incubate at 4 °C overnight on rotary mixer. Add 80 µl N-ethyl maleimide (0.1 mM) to reaction mixture to stop reaction. Agitate and incubate 30 mins at room temperature Concentrate to 0.5 ml or less on Centricon
, Ethyl acetate (EtOAc) Petroleum ether (b.p. range, 40° to 80°C) Isopropanol (i ‐PrOH
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