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- CAS号：
34590-94-8
Dipropylene Glycol Monomethyl Ether (Mixture of Isomers)
34590-94-8
C₇H₁₆O₃
148.2
Colourless Oil
Chloroform (Slightly), Methanol (Slightly)
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文献和实验Dipropylene Glycol Monomethyl Ether (Mixture of Isomers)
34590-94-8
C₇H₁₆O₃
148.2
Colourless Oil
Chloroform (Slightly), Methanol (Slightly)
Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
Isolation of Microtubules (Bovine Brain)
)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis( -aminoethyl Ether) N,N,N',N'-tetraacetic acid) 0.5 mM MgCl Adjust pH to 6.4 8 M Glycerol in MT buffer Virtis homogenizer
by the addition of polyethylene glycol and collected by centrifugation. The DNA pellet is resuspended in a buffer containing cesium chloride and ethidium bromide, which is loaded into polyallomer tubes and subjected to ultracentrifugation overnight. The ethidium
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