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- CAS号：
146368-15-2
Cy 5 (Technical Grade)
146368-15-2
C₄₅H₅₂N₄O₁₄S₂
937.04
Red to Very Dark Purple Sticky Solid to Solid
Methanol (Slightly), Water (Slightly)
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文献和实验Cy 5 (Technical Grade)
146368-15-2
C₄₅H₅₂N₄O₁₄S₂
937.04
Red to Very Dark Purple Sticky Solid to Solid
Methanol (Slightly), Water (Slightly)
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
Cyanine Dye Purification Protocol
Slowly add 111.3 mL (80.95 g) HPLC grade triethylamine; the reaction is exothermic; the solution will become cloudy � wait until it clears before adding more) Add dropwise an additional 10-15 mL acetic acid to adjust the pH to 8
Optimizing the Difference Gel Electrophoresis (DIGE) Technology
Difference gel electrophoresis (DIGE) technology has been used to provide a powerful quantitative component to proteomics experiments involving 2D gel electrophoresis. DIGE combines spectrally resolvable fluorescent dyes (Cy2, Cy3, and Cy
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