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文献和实验Isolation of Recombinant DNA Elongation Proteins
This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) δ, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d
Identification of Protein Interactions by Far Western Analysis
Basic Protocol 1: Far Western Analysis of a Protein Mixture Alternate Protocol 1: Detecting Interacting Proteins by Immunoblotting Alternate Protocol 2: Using Peptides to Identify Specific
The STINT‐NMR Method for Studying In‐cell Protein‐Protein Interactions
. Slade, K.M., Baker, R., Chua, M., Thompson, N.L., and Pielak, G.J. 2009. Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli. Biochemistry 48:5083‐5089
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