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文献和实验Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase
In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification
Analysis of Human Mitochondrial DNA Mutations
their own genetic material—mitochondrial DNA (mtDNA)-mitochondria are unique mammalian organelles. Normal human mtDNA is a 16,569 base-pair (bp), double-stranded, circular molecule ( 1 ). The molecules contain tightly compacted genes for 22 transfer (
Construction of Recombinant Human Cytomegalovirus
The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral
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