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文献和实验Overview of Protein Microarrays
: Applications to signalling pathways and human body arrays. Brief. Funct. Genom. Proteom. 1:305‐315. Chen, C.‐S. and Zhu, H. 2006. Protein microarrays. BioTechniques 40:423‐429
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
on engineered aminopeptidases. Using human tumor necrosis factor α as a model protein, we describe here the steps involved in the removal of a His-tag using TAGZyme. The tag used (UZ-HT15) has been optimized for expression in Escherichia coli and for TAGZyme
properties. In 1985 two distinct, but distantly related complementary DNAs encoding proteins sharing human IL-1 activity were reported to be isolated from a macrophage cDNA library, thus defining two individual members of the IL-1 family - IL-1α and IL
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