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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Recommended Refrigeration Conditions (2℃-8℃) for storage
- 英文名:
2-[(2S)-2-piperidyl]ethanol
- 供应商:
南京鼎石
- CAS号:
103639-57-2
- 规格:
10g///25g///50g///100g///250g///500g///1kg///5kg///29072kg
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文献和实验Decant the supernatant rapidly Wash 2× with ice cold PBS (800 rpm for 5 min at 4℃) Resuspend the pellet in the residual PBS, pipet it into 2 ml of 90% ethnol/10% PBS (-20℃ ) (15 ml tube) and vortex for 1-2s Incubate cell suspention for 20 min
是去垢的作用; PMSF PMSF即Phenylmethanesulfonyl fluoride,也称Phenylmethylsulfonyl fluoride或α-Toluenesulfonyl fluoride。中文名为苯甲基磺酰氟。分子式为C7H7FO2S,分子量为174.19。可抑制丝氨酸蛋白酶例如胰蛋白酶(trypsin)和胰凝乳蛋白酶(chymotrypsin),也可抑制半胱氨酸蛋白酶和乙酰胆碱酯酶。有效浓度为0.1-1mM。难溶于水,且在水溶液中非常不稳
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
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