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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Protected by Nitrogen (2℃-8℃)
- 英文名：
5-chloro-6-methoxy-1h-indole
- 供应商：
南京鼎石
- CAS号：
90721-60-1
- 规格：
10g///25g///50g///100g///250g///500g///1kg///5kg///25531kg
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文献和实验X-gal (abbreviated to BCIG) is a galactoside and indole. 也称5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside或5-Bromo-4-chloro-3-indolyl β-D-galactoside，中文名为5-溴-4-氯-3-吲哚-β-D-半乳糖
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
Who can help me-Molecular Biology
and incubate 55� ON. - phenol/chloro extraction 1h @ 4℃ under soft rotation. - chloro/isoamyl alcool (24:1) - 1 Vol Isopropanol precipitation. Maybe I use a higher conc of proteinase K than you...I don't know, but I think it is a protein contamination
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