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- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Recommended Refrigeration Conditions (2℃-8℃) for storage
- 英文名：
1-(4-bromophenyl)-2-methyl-propane-1,2-diol
- 供应商：
南京鼎石
- CAS号：
612070-23-2
- 规格：
10g///25g///50g///100g///250g///500g///1kg///5kg///17376kg
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文献和实验deionized formamide 500 m l 10X TBE 500 m l 0.2% bromophenyl blue and 0.2% xylene cyanol Other Reagents Needed: 0.22 m M disposable syringe filters, short wave UV source, intensifying screen for UV shadowing Procedure • Pour a 20% denaturing gel: 12 ml
Protein Gel Preparation and Staining
250 mM Tris Base 1.65 g Tris Base 250 mM glycine 9.35 g glycine 0.1% SDS 2.5 ml 20% SDS up to 500 ml with Q 5X Loading Dye 0.5 M Tris 6.8 5 ml 1M Tris pH 6.8 50% glycerol 5 ml glycerol 0.5% bromophenyl blue 0.05 g
In vitro transcription with yeast nuclear extract
). Incubate 3 min at room temp. 6. Add 3 microliters sequencing gel loading buffer (formamide/EDTA plus bromophenyl blue and xylene cyanol) and mix well. 7. Heat 1 min at 90 - 100 degrees and immediately put on ice. 8. Load to 6% DNA sequencing gel and run
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