{2-oxabicyclo[2.2.2]octan-4-yl

原创：western protocol

Tris(PH=6.8) 10% SDS 40 µl 10% ammonium persulfate 40 µl TEMED 7 µl C. Preparation of gel 1.  Assemble the glass plates and spacers (0.75 mm thick). 2.  Pour the running gel to about 1 cm below the wells of the comb (~3.2 ml). 3.  Seal with 1 ml

发挥抗体极致效能 达人都在做的免疫印迹法

制备蛋白提取物1. Prepare extracts from cultured cells or tissues with our Trident Extraction Kits.The total number of cells per ml and the cell equivalent loaded per lane of gel should be optimized specifically for each protein and antibody.2. Determine

Gonad Fixation

than methanol by itself. To make the fixative, mix 10 mls of 16% formaldehyde, 3.3 mls of 0.1 M K2 HPO4 (pH7.2), and 40 ml methanol. This solution is stored at -20¡ and is used as described for methanol (but increase fixation time to 10 min).  D