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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Recommended Refrigeration Conditions (2℃-8℃) for storage
- 英文名:
1H-imidazole-2-carbonitrile
- 供应商:
南京鼎石
- CAS号:
31722-49-3
- 规格:
10g///25g///50g///100g///250g///500g///1kg///5kg///10748kg
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
buffer: 6 to8M Urea , 20mM Tris pH 7.5, 100mM NaCl, and appropriate imidazole concentrations. Limitations Do not exposed Ni matrices to reducing agents as DTT or DTE ( you can use ßME up to 20mM); chelating agents as EDTA and EGTA; NH
,1ml NI-NTA/柱),待完全沉淀(约1h),先用10ml灭菌水洗,流速:重力作用 2.6 10ml 1*Bind Buffer 进行平衡,流速:重力作用 2.7 将准备好的样品上样,pET.wap.ns; PET21 only分别上柱(结合时间1h-1.5h流速控制在0.1ml/min左右, EP 管,分管收集上样后液体) 2.8 10ml 1* Bind Buffer 洗柱子(收集洗脱液10管,流速在0.3-0.4ml/min左右)
for the etherification at the O 6 ?position of silyl?protected inosine, guanosine, and 2??deoxyguanosine is described. Typically, a THF solution of the silylated nucleoside is treated with 1H ?benzotriazol?1?yloxy?tris(dimethylamino)phosphonium hexafluorophosphate (BOP
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