KRAS [G12R] Avi&His Tag Protei

Immunoprecipitation and Phosphorylation of G Protein-Coupled Receptors

G protein-coupled receptors (GPCRs) are integral membrane proteins with seven transmembrane-spanning α-helices. Following ligand activation, many GPCRs are rapidly phosphorylated on serine/threonine residues in their cytoplasmic domains

Overview of Formation of G‐Quadruplex Structures

  Figure 17.2.3 Folding topology of the human telomeric G‐quadruplex formed by (A ) d[AG3 (T2 AG3 )3 ] in Na+ solution, (B ) d[AG3 (T2 AG3 )3 ] in K+ crystal structure, (C ) d[TAG3 (T2 AG3 )3 ] in K+ solution

干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密

CUT&Tag（Cleavage Under Targets and Tagmentation）是蛋白-DNA 互作的一大革新技术，它不需要使用甲醛交联以及免疫共沉淀，而是通过针对靶蛋白（如转录因子、染色质重塑蛋白）的抗体和 Protein A/G 的介导，使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头，经 PCR 扩增后即可形成用于高通量测序的文库（见图 1 ）。CUT &Tag 技术具有细胞投入量低、信噪