Recombinant mouse Htra1 (HEK29

Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes

with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles

Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of Their Protein Function

1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable

Genotyping from mouse tails

Genotyping from mouse tails   Chen-Ming Fan Lab,Carnegie Institute of Washington (Laura’s method) All pipets must be filter tipped Be very careful to prevent cross contamination of samples Change gloves often