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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology
transfected cells. This will reduce the non-physiological interaction due to overexpression and allow time for YPI-OneStrep to be incorporated into even highly stable complexes. After transfection of HeLa S3 cells with plasmids carrying the OneStrep fused to N
Introduction of human 14-3-3 proteins
regulation.We report here the crystal structure of the human T-cell 14-3-3 isoform (tau)dimer at 2.6 A resolution.Each monomer (Mr 28K)is composed of an unusual arrangement of nine antiparallel alpha-helices organized as two structural domains.The dimer
Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
in intracellular Ca2+ levels and PKC translocation to the plasma membrane in response to mGluR1 stimulation in transiently transfected human embryonic kidney (HEK293) cells. The changes in intracellular Ca2+ were imaged using the fluorescent indicator Oregon Green
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