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文献和实验Conclusions These data demonstrate the expression of a biologically active human recombinant paraoxonase-1 protein in the mammalian cell line CHO as shown by the capacity to dephosphorylate para-nitrophenyl phosphate. The protein
and corresponding protein levels to correlate phenotypic changes--monitored by enzymatic assay, cell based assay, gene expression profiling, or other means--with extent of knockdown induced by an siRNA. Advantages of TaqMan Gene Expression Assays qRT-PCR provides
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
just prior to experimentation using cell?free expression systems. As such, the NAPPA platform offers a unique and viable alternative that circumvents many of the inherent limitations of spotted protein arrays, enabling diverse functional protein studies including protein?small
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