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- 2025年07月09日
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文献和实验Potentiated autologous tumor cell and peripheral blood lymphocyte lysate vaccination
-term cultures from the patient who is to be vaccinated. The culture medium (RPMI 1640) is free of fetal calf serum (FCS). The coculture is mixed with autologous plasma in a 1:1 ratio with the addition of 200 IU of recombinant human interleukin-2/mL
active form of human recombinant PON-1 in the mammalian cell line CHO. METHODS Construction of the paraoxonase : cloning vector The coding region for human paraoxonase-1 was amplified from human liver cDNA using a set of gene specific
A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus
to be about 0.1% (for a review, see ref. 3 ). In the original protocol for the isolation of a recombinant vaccinia virus (6 ), cell monolayers are infected with the wild-type virus (WR strain) and transfected with the plasmid DNA encoding the gene of interest. Since insertion
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