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文献和实验active form of human recombinant PON-1 in the mammalian cell line CHO. METHODS Construction of the paraoxonase : cloning vector The coding region for human paraoxonase-1 was amplified from human liver cDNA using a set of gene specific
Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
in intracellular Ca2+ levels and PKC translocation to the plasma membrane in response to mGluR1 stimulation in transiently transfected human embryonic kidney (HEK293) cells. The changes in intracellular Ca2+ were imaged using the fluorescent indicator Oregon Green
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
have been isolatedfrom whole tissue or cell culture (5 , 6) , relatively small quantitieshave been obtained in this manner. There are no reportsof recombinant expression in prokaryotic systems, presumablybecause of the folding problems associated with production
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