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文献和实验Potentiated autologous tumor cell and peripheral blood lymphocyte lysate vaccination
-term cultures from the patient who is to be vaccinated. The culture medium (RPMI 1640) is free of fetal calf serum (FCS). The coculture is mixed with autologous plasma in a 1:1 ratio with the addition of 200 IU of recombinant human interleukin-2/mL
active form of human recombinant PON-1 in the mammalian cell line CHO. METHODS Construction of the paraoxonase : cloning vector The coding region for human paraoxonase-1 was amplified from human liver cDNA using a set of gene specific
1:膜蛋白应用实例 蛋白表达系统纯度产率 (所有回收蛋白)采取的纯化步骤hCB2 G蛋白偶联受体大肠杆菌90%4.5mg/5L 培养物Rho1D4 IAC+SEChVN1R1 G蛋白偶联受体可诱导的HEK293S细胞系(哺乳动物)90%1mg/1g细胞Rho1D4 IAC+SECFPR3 G蛋白偶联受体可诱导的HEK293S细胞系(哺乳动物)90%2mg/6g细胞Rho1D4 IAC+SECTAAR5 G蛋白偶联受体可诱导的HEK293S细胞系(哺乳动物)90
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