Recombinant Human Alpha-crysta

Recombinant Single Globin-Chain Expression and Purification

of normal and modified human globin chains in bacteria employing a fusion-protein expression vector (1 ). An expression system was later developed in which α- and β-globin chains were coexpressed, resulting in the formation of soluble tetrameric Hb in yeast

Expression Cloning of Human B Cell Immunoglobulins

characterization of the human antibody repertoire on a single cell level through the generation of recombinant monoclonal antibodies from single primary human B cells of defined origin. This protocol offers a detailed description of the method starting

Human In-Cell scFv Library from Infiltrating B Cell

The construction of a large library of single-chain Fv (scFv) antibody fragments involves a random assortment of heavy and light chains. Although useful for the production of recombinant antibodies, this method is not totally adapted