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文献和实验Expression of Tetanus Toxin Fragment C
The Pichia pastoris system has now been used successfully to express a large number of different intracellular and secreted proteins. In some cases, extremely high levels have been obtained (e.g., tumor necrosis factor, TNF [1 ]; human serum
Bordetella pertussis , the causative agent of whooping cough, secretes a plethora of virulence factors contributing to the onset and characteristic of the disease. The exotoxin pertussis toxin (PT) is regarded as particularly important
Making antibodies to synthetic peptides
to tyr), EDAC (carbodiimide; couples amino to carboxyl). We often add an extra ("adventitious") tyr to the N- or C-terminus of the synthetic peptide, for BDB coupling. We've done this more often than not. Try to put the extra tyr
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