Der F1 Mosaic Protein Recombin

Preparation of recombinant fibronectin fragments for functional and structural studies

integrin receptors. The mosaic nature of fibronectin permits it to be analyzed by a “dissection” strategy, where protein fragments generated by recombinant expression in E. coli , P. pastoris , and human cell lines are employed in structural and functional

Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonan

that their polypeptide components are frequently comprised of a series of sequence repeats or “modules” (1 ). This mosaic nature of ECM macromolecules permits their properties to be analyzed by a “dissection” strategy (2 ), where protein fragments generated

Secretion of the Respiratory Syncytial Virus Fusion Protein From Insect Cells Using the Baculovirus Expression System

. The secreted recombinant GST-F2 /F1 protein was further analysed using glycosidases. Our results showed that the GST-F2 /F1 protein were sensitive to peptide: N -glycosidase F (PNGase F) treatment, but not to Endoglycosidase H (EndoH) treatment. This indicates