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文献和实验Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true
Human In-Cell scFv Library from Infiltrating B Cell
to the study of the antibody repertoire formed in vivo during, for example, autoimmune diseases. We describe here, the use of the in-cell PCR together with Cre-recombination applied to human B cells to obtain in situ pairing of the variable (V
Expression Cloning of Human B Cell Immunoglobulins
characterization of the human antibody repertoire on a single cell level through the generation of recombinant monoclonal antibodies from single primary human B cells of defined origin. This protocol offers a detailed description of the method starting
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