Janelia Fluor 549 conjugation

Overview

The Hello Bio Janelia Fluor® 549 conjugation kit allows the conjugation of antibodies and proteins to Janelia Fluor® 549 in as little at 90 minutes (15 minutes active time) with a high degree of labeling. There are many benefits of directly labeled proteins and antibodies such as:

• Much easier multiplexing - no need to mix and match antibody species correctly
• Avoid non-specific binding by secondary antibodies
• Save time by shortening staining protocols.

Requirements

• Not compatible with BSA containing antibodies or proteins as this will reduce the taget labeling. These should be removed before processing.
• The protein to be labeled should be greater than 7kDa in size
• The antibody concentration should be at least 1mg/ml, lower concentrations can be used however this will effect the degree of labeling.

Pack size guidance

Please note:

• The 2x50µg packsize is sufficient to carry out 2 conjugation reactions on 50µg protein each.
• The 3x100µg packsize is sufficent to carry out 3 conjugation reactions on 100µg protein each.