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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-Npap60 / Nup50 Antibody
- 抗体英文名:
Anti-Npap60 / Nup50 Antibody
- 浓度:
/
- 应用范围:
Western blot, Paraffin-embedded IHC
- 宿主:
Goat
- 克隆性:
/
- 保存条件:
-20°C
- 亚型:
/
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
标本。 2. 加20ul 1 N HC I ,盖紧,上下混匀。 2 - 8 ℃ 放置60± 2 分钟。 3. 加20ul 1 N NaOH, 盖紧,上下混匀。 4. 即用,或放-20/-70 ℃ 保存3 天。计算结果时乘 以稀释倍数 50 。 ( 注意:不同的标本E2F1 的水平可能有较大差异,请根据实际情况灵活掌握稀释度) 5. 细胞培养上清或组织匀浆 10 倍稀释 (410ul 的标本
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