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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-p53 / TP53 (C-Terminal Region) Antibody, Sodium Azide Free
- 抗体英文名:
Anti-p53 / TP53 (C-Terminal Region) Antibody, Sodium Azide Free
- 浓度:
1 mg/ml in 1X PBS, BSA free, sodium azide free
- 应用范围:
Western blot: 0.5-1ug/ml IHC (Frozen) only: 0.5-1ug/ml for 30 min at RT
- 宿主:
Mouse
- 克隆性:
/
- 保存条件:
≤-20°C
- 亚型:
IgG2b, kappa
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文献和实验Purification Of Anti Peptide Antibody
the antibody can be concentrated by sweating the dialysis bags, or by spin-concentrating. Bring the azide concentration up to 0.1 and store at 4 deg C for up to three months. For longer storage freeze in aliquots and store at -80 degC or add glycerol
Purification Of Anti Peptide Antibody
Purification Of Anti Peptide Antibody Claire Walczak January 1996 Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C
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