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- RayBio
- 188-11044
- 2025年07月09日
- FACS: 0.5-1ug/10e6 cells in 0.1ml Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-Glypican 3 / GPC3 Antibody
- 抗体英文名：
Anti-Glypican 3 / GPC3 Antibody
- 浓度：
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
- 应用范围：
FACS: 0.5-1ug/10e6 cells in 0.1ml Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
- 宿主：
Rabbit
- 克隆性：
/
- 保存条件：
≤-20°C
- 亚型：
IgG
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文献和实验Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Purification Of Anti Peptide Antibody
Purification Of Anti Peptide Antibody Claire Walczak January 1996 Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls
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