- ￥99999
- RayBio
- 188-10663
- 2025年07月13日
- Flow Cytometry: 0.5-1ug/million cells in 0.1ml Prediluted format: incubate for 30 min at RT (2)
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-Alpha Smooth Muscle Actin Antibody
- 抗体英文名：
Anti-Alpha Smooth Muscle Actin Antibody
- 浓度：
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
- 应用范围：
Flow Cytometry: 0.5-1ug/million cells in 0.1ml Prediluted format: incubate for 30 min at RT (2)
- 宿主：
Mouse
- 克隆性：
/
- 保存条件：
≤-20°C
- 亚型：
IgG2a, kappa
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
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