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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-Mouse IL7R / CD127 (pTyr449) Antibody
- 抗体英文名:
Anti-Mouse IL7R / CD127 (pTyr449) Antibody
- 浓度:
1 mg/ml
- 应用范围:
ELISA (1:100000), IHC-P (5 µ,g/ml), Western Blotting (1:1000 - 1:10000)
- 宿主:
Rabbit
- 克隆性:
/
- 保存条件:
-20°C
- 亚型:
/
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文献和实验Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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