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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-KIAA0152 / MLEC (Internal) Antibody
- 抗体英文名:
Anti-KIAA0152 / MLEC (Internal) Antibody
- 浓度:
1 mg/ml
- 应用范围:
IHC-P (2.5 µ,g/ml)
- 宿主:
Rabbit
- 克隆性:
/
- 保存条件:
-70°C
- 亚型:
/
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文献和实验Protocol for anti-HA antibody Western Blotting
film for ~2 mins. Notes on the primary antibody: Source : Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in -20℃ Internal notes: labelled anti
Protocol for anti-HA antibody Western Blotting
it around for 5-10 seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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