- ￥3841
- RayBio
- 102-25370
- 2025年07月13日
- Western Blotting
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-CREB (S133) Antibody
- 抗体英文名：
Anti-CREB (S133) Antibody
- 浓度：
/
- 应用范围：
Western Blotting
- 宿主：
Rabbit
- 克隆性：
Polyclonal
- 保存条件：
-20°C
- 亚型：
Rabbit, kappa
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文献和实验which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
a simple ELISA method to detect human anti-antibody response.
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