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- RayBio
- 102-10185
- 2025年08月27日
- Western Blotting
- 0
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-MME/CD10 (ascites) Antibody
- 抗体英文名：
Anti-MME/CD10 (ascites) Antibody
- 浓度：
/
- 应用范围：
Western Blotting
- 宿主：
0
- 克隆性：
Monoclonal
- 保存条件：
-20°C
- 亚型：
IgG1, kappa
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文献和实验Purification of Antiserum or Ascites by Protein A/G Chromatography
PreparationTo prepare 1 liter of TBS (50 mM Tris-HCl, pH 7.4; 150 mM NaCl; 0.05% sodium azide) add the following to 800ml of distilled water:Ⅰ6.06 g of Tris baseⅡ8.77 g of NaClⅢ 10ml of 5% sodium azide stock solution (Do not add if the antibody will used
to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media
) (1 ,2 ). In these procedures, the wells of 96-well plates or strip plates are coated with antigen. Antibody that binds to the antigen is detected by its reaction with an anti-immunoglobulin that has been conjugated to a reporter molecule, usually horseradish peroxidase (HRP
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