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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见斯达特(Starter)网站
- 英文名:
Biotinylated CD40L/CD154/TRAP/gp39 His&Avi tag Protein,Human
- 库存:
999
- 供应商:
斯达特生物
- 规格:
25μg
产品货号: UA011158
产品名称: Biotinylated CD40L/CD154/TRAP/gp39 His&Avi tag Protein,Human
中文名称: 生物素化 CD40L/CD154/TRAP/gp39 His&Avi 标记蛋白,Human
产品分类: 药物靶点
宿主来源: Human
抗原名称/别名: CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein (TRAP), Tumor necrosis factor ligand superfamily member 5, CD154, CD40LG, CD40L, TNFSF5, TRAP
Accession: P29965
方法学应用: 见斯达特生物网站
反应种属: 见斯达特生物网站
标记: Avi Tag, His Tag
缓冲体系: Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
表达系统: HEK293
储存条件:
12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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文献和实验Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
function. Many R proteins are associated with the plasma membrane (PM) and expressed at low levels. Here, we describe a method to purify such low-abundance PM R protein �complexes from Arabidopsis using a biotinylated affinity tag, called the HPB tag
Western Blotting with Biotinylated Antibodies
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
Western Blotting with Biotinylated Antibodies
Western Blotting with Biotinylated Antibodies SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline
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