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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白的浓度
- 适应物种:
大鼠
- 标记物:
Rat Serine protease inhibitor A3L,Serpina3l
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.78pg/ml
- 规格:
96tests
Rat Serine protease inhibitor A3L,Serpina3l ELISA试剂盒
Product Name:Rat Serine protease inhibitor A3L,Serpina3l ELISA试剂盒
Packing:96T
Catalog No.:Rat Serine protease inhibitor A3L,Serpina3l ELISA试剂盒
Gene Name:Rat Serine protease inhibitor A3L,Serpina3l
Detect Range:78-5000pg/mL
Sensitivity:46.79pg/mL
Target Protein Name:Rat Serine protease inhibitor A3L,Serpina3l
Alternative Name:Rat Serine protease inhibitor A3L,Serpina3l
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat Serine protease inhibitor A3L,Serpina3l ELISA KIT allows for the in vitro quantitative determination of Rat Serine protease inhibitor A3L,Serpina3l concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rat Serine protease inhibitor A3L,Serpina3l ELISA KIT has been pre-coated with an Rat Serine protease inhibitor A3L,Serpina3l antibody specific to Rat Serine protease inhibitor A3L,Serpina3l .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat Serine protease inhibitor A3L,Serpina3l and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Serine protease inhibitor A3L,Serpina3l, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat Serine protease inhibitor A3L,Serpina3l in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【求助】求助!!关于EMSA核蛋白提取过程中用到的100x Protease Inhibitor
要购买,而分别购买这些试剂的钱加起来可以买个核蛋白提取试剂盒了很不划算。有没有做EMSA的人啊,你们提核蛋白的Protease Inhibitor都怎么来的? 有人用过上海生工的核蛋白提取试剂盒了,那个只要250元,提出来的效果怎么样? xhjggl 效果比较好,他们也有100x Protease Inhibitor卖 ,打折卖100x1ml 525元 月下独见 谢谢LS,这样看直接买核蛋白提取试剂盒还划算
实验试剂 1. Aluminum Foil 2. Liquid Nitrogen 3. Dry Ice 4. Phosphate Buffered Saline 5. Protease Inhibitor Cocktail (Sigma Cat.no. P-8340)
) or early death. The serine protease uPA, in concert with its inhibitor PAI-1, promotes tumor cell adhesion, migration, and proliferation, as well as extracellular matrix degradation and, thus, facilitates tumor cell invasion and metastasis. The various
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