- ￥3280
- LSMBIO
- 78-5000pg/ml
- 46.78pg/ml
- 进口
- ELI-11946r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白的浓度
- 适应物种：
大鼠
- 标记物：
Rat RAC-gamma serine/threonine-protein kinase,Akt3
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.78pg/ml
- 规格：
96tests
Rat RAC-gamma serine/threonine-protein kinase,Akt3 ELISA试剂盒
Product Name:Rat RAC-gamma serine/threonine-protein kinase,Akt3 ELISA试剂盒
Packing:96T
Catalog No.:Rat RAC-gamma serine/threonine-protein kinase,Akt3 ELISA试剂盒
Gene Name:Rat RAC-gamma serine/threonine-protein kinase,Akt3
Detect Range:78-5000pg/mL
Sensitivity:46.79pg/mL
Target Protein Name:Rat RAC-gamma serine/threonine-protein kinase,Akt3
Alternative Name:Rat RAC-gamma serine/threonine-protein kinase,Akt3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat RAC-gamma serine/threonine-protein kinase,Akt3 ELISA KIT allows for the in vitro quantitative determination of Rat RAC-gamma serine/threonine-protein kinase,Akt3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rat RAC-gamma serine/threonine-protein kinase,Akt3 ELISA KIT has been pre-coated with an Rat RAC-gamma serine/threonine-protein kinase,Akt3 antibody specific to Rat RAC-gamma serine/threonine-protein kinase,Akt3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat RAC-gamma serine/threonine-protein kinase,Akt3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat RAC-gamma serine/threonine-protein kinase,Akt3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat RAC-gamma serine/threonine-protein kinase,Akt3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验起来以检测蛋白的数量;阳性和阴性对照也需要使用在试剂盒中GTP和 GTP-gamma-S reagents进行准备.然后将PAK-1 PBD beads加入到细胞裂解液中(10 ul of beads for every 500 ul of cell lysate).接着孵育以保证Rac1-GTP的结合，使用buffer 清洗the beads以去除细胞碎片和没有结合的Rac1。 最后，加入 laemmli buffer (not provided)到 beads，将泥浆使用anti-Rac
Identification and Functional Reconstitution of Effector Proteins for the GTPases Rac and CDC42HS
Aldrich Syndrome Protein (WASP) ( 7 , 8 ). Rho was recently shown to interact with several protein kinases related to protein kinase N ( 9 , 10 ). Fig. 1. Cycling between the “on” and “off” states of Ras-like GTPases is regulated by GEFs
Angiotensin Protein Kinase Assay
James Hardwick's angiotensin assay protocol This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviously use fewer or more cells, depending
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