Avian leukosis virus

平台编号：bio-84425
Avian leukosis virus 拉丁名

(ATCC® VR-1828™) 编号

Classification Retroviridae, Alpharetrovirus, Avian leukosis virus (Subgroup B) 

Deposited As Rous-associated virus-B 

Agent Avian leukosis virus 

Strain 菌株别名 RAV-2 

Biosafety Level 安全等级 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 1.0 mL 

Storage Conditions 培养条件 

-70°C or colder 

Images VR-1828 images 

Comments 注释 Derived by adaptation from primary cell culture product ATCC® VR-658™ to UMNSAH/DF-1 cells (ATCC® CRL-12203™).  ATCC® VR-658™ and ATCC® VR-1828™ have not been compared for sequence or infectivity in primary cell culture and continuous tissue culture. 

Effect on Host CPE, cell enlargement and vacuolization. May also cause syncytia or cell rounding and sloughing. Recommended Host UMNSAH/DF-1 cells (ATCC® CRL-12203™) 

Growth Conditions 培养条件

Temperature 培养温度 : 37°C 

Recommendations for Infection: For best results, infection 

should be performed on a 60-80% confluent, 18-48 hour old 

cellular monolayer. Prepare dilution of virus in minimum amount of 

volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or 

serum free medium prior to inoculation. Adsorb virus dilution for 1-

2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking 

every 20-30 minutes to redistribute inoculum. End adsorption by 

adding virus growth medium. 

Incubation: 7-14 days at 37°C in a humidified 5% CO2 

atmosphere, until CPE is progressed through 80% of the 

monolayer.  Media changes may be necessary. 

Effect on Host CPE, cell enlargement and vacuolization. May also cause syncytia or cell rounding and sloughing. 

Name of Depositor 寄存人 W Okazaki, HG Purchase, Holdenried, E Berstein 

References 参考文献 Morgan HR, Traub W. Natl. Cancer Inst. Monogr. 17: 392, 1964.

Vogt PK. Envelope classification of avian RNA tumor viruses. Bibl. Haematol. 36: 153-167, 1970. PubMed: 4376359 

Vogt PK. A heterogeneity of Rous Sarcoma Virus revealed by selectively resistant chick embryo cells. Virology 25: 237-247, 1965. PubMed: 14297211

Himly M, et al. The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses. Virology 248: 295-304, 1998. PubMed: 9721238

Schaefer-Klein J, et al. The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors. Virology 248: 305-311, 1998. PubMed: 9721239 

Gao Q et al. Development and application of a multiplex PCR method for rapid differential detection of subgroup A, B, and J avian leukosis viruses. J. Clin. Microbiol. 52(1): 37-44, 2014. PubMed: 24131697