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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测小鼠血清,血浆,组织匀浆等样本中目的蛋白的含量
- 适应物种:
小鼠
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
46.79pg/ml
- 规格:
96tests
Mouse AF9 (Protein AF-9) ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Mouse AF9 ELISA KIT ,Mouse Protein AF- 9 ELISA KIT ,Mouse MLLT3 ELISA KIT ,Mouse YEATS3 ELISA KIT ,Mouse ALL1-fused gene from chromosome 9 protein ELISA KIT ,Mouse YEATS domain-containing protein 3 ELISA KIT ,Mouse myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog) ELISA KIT ,Mouse translocated to 3 ELISA KIT ,Mouse myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog Drosophila) ELISA KIT ,Mouse translocated to 3 ELISA KIT ,Mouse myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein ELISA KIT ,Mouse myeloid/lymphoid or mixed-lineage leukemia ELISA KIT ,Mouse translocated to 3 ELISA KIT ,Mouse myeloid/lymphoid or mixed-lineage leukemia ELISA KIT ,Mouse translocated to 3 ELISA KIT
Intended use
Mouse AF9 ELISA kit allows for the in vitro quantitative determination of Mouse Protein AF-9 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse AF9 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AF9, During the reaction, AF9 in the sample or standard competes with a fixed amount of biotin-labeled AF9 for sites on a pre-coated Monoclonal antibody specific to AF9. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AF9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
Order Mouse AF9 ELISA kit by email:sales@
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文献和实验Transgenic Mouse and Transgenic Rat Outline
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ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure 1. All incubations
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