Mouse Growth arrest and DNA da

Mouse Growth arrest and DNA damage- inducible protein GADD45 gamma, GADD45G ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

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Intended use

Mouse GADD45G ELISA kit allows for the in vitro quantitative determination of Mouse GADD45G concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Mouse GADD45G ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GADD45G and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GADD45G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GADD45G in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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