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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测小鼠血清,血浆,组织匀浆等样本中目的蛋白的含量
- 适应物种:
小鼠
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
46.79pg/ml
- 规格:
96tests
Mouse Melanocyte protein PMEL, PMEL ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse PMEL ELISA KIT ,Mouse melanocyte protein Pmel 17 ELISA KIT ,Mouse D12S53E ELISA KIT ,Mouse ME20 ELISA KIT ,Mouse ME20-M ELISA KIT ,Mouse ME20M ELISA KIT ,Mouse P100 ELISA KIT ,Mouse PMEL17 ELISA KIT ,Mouse SIL ELISA KIT ,Mouse SILV ELISA KIT ,Mouse gp100 ELISA KIT ,Mouse melanocyte protein mel 17 ELISA KIT ,Mouse melanocytes lineage-specific antigen GP100 ELISA KIT ,Mouse melanoma-associated ME20 antigen ELISA KIT ,Mouse melanosomal matrix protein17 ELISA KIT ,Mouse silver locus protein homolog ELISA KIT ,Mouse silver ELISA KIT ,Mouse mouse ELISA KIT ,Mouse homolog of ELISA KIT ,Mouse premelanosome protein ELISA KIT
Intended use
Mouse PMEL ELISA kit allows for the in vitro quantitative determination of Mouse PMEL concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse PMEL ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PMEL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PMEL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PMEL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse PMEL ELISA kit Range 78-5000pg/ml
<":////Human-Melanocyte-protein-PMEL-PMEL-ELISA-Kit">Mouse PMEL ELISA kit manual
Order Mouse PMEL ELISA kit by email:sales@
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文献和实验ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure 1. All incubations
minutes. Add 100 µL/well stop solution (0.5 M Oxalic Acid). Read absorbance at 414 nm in an ELISA reader. 相关仪器试剂设备 酶标仪 洗板机 化学发光检测仪 ELISA检测试剂盒 ELISPOT检测 ELISA试剂盒 抗体 酶标板
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