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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测小鼠血清,血浆,组织匀浆等样本中目的蛋白的含量
- 适应物种:
小鼠
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
46.79pg/ml
- 规格:
96tests
Mouse CCN family member 5, CCN5 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
WISP2,CCN5; CT58; CTGF-L; CCN family member 5; WISP-2; connective tissue growth factor-like protein; connective tissue growth factor-related protein 58; wnt-1 signaling pathway protein 2; WNT1 inducible signaling pathway protein 2
Search name
Mouse CCN5 ELISA KIT , Mouse WISP2 ELISA KIT ,Mouse CT58 ELISA KIT ,Mouse CTGF-L ELISA KIT ,Mouse CCN family member 5 ELISA KIT ,Mouse WISP-2 ELISA KIT ,Mouse connective tissue growth factor-like protein ELISA KIT ,Mouse connective tissue growth factor-related protein 58 ELISA KIT ,Mouse wnt-1 signaling pathway protein 2 ELISA KIT ,Mouse WNT1 inducible signaling pathway protein 2 ELISA KIT
Intended use
Mouse CCN5 ELISA KIT allows for the in vitro quantitative determination of mouse CCN family member 5,CCN5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Mouse CCN5 ELISA KIT has been pre-coated with an antibody specific to CCN5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CCN5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CCN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CCN5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse CCN5 ELISA KIT Range 0.156-10ng/ml
Mouse CCN5 ELISA KIT <":////Mouse-CCN-family-member-5-CCN5-ELISA-Kit">
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文献和实验secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
was unresponsive to the transcription factors (Fig. 4D). The sequence 5'-GGAA-3' (ETS-binding site-2, EBS2) is conserved between mouse and the human promoters, and in the mouse it interacts with the ETS-1 protein, a transcription factor of the ETS family expressed
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