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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测小鼠血清,血浆,组织匀浆等样本中目的蛋白的含量
- 适应物种:
小鼠
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
46.79pg/ml
- 规格:
96tests
Mouse Thrombin-antithrombin complex, TATC ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TATC,Thrombin-antithrombin complex, Thrombin-antithrombin,TAT, Thrombin, antithrombin
Search name
Mouse TATC ELISA KIT ,Mouse Thrombin-antithrombin complex ELISA KIT ,Mouse Thrombin-antithrombin ELISA KIT ,Mouse TAT ELISA KIT ,Mouse Thrombin ELISA KIT ,Mouse antithrombin ELISA KIT
Intended use
Mouse TATC ELISA KIT allows for the in vitro quantitative determination of mouse Thrombin-antithrombin complex concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Mouse TATC ELISA KIT has been pre-coated with an antibody specific to Thrombin-antithrombin complex. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Thrombin-antithrombin complex and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Thrombin-antithrombin complex, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thrombin-antithrombin complex in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 0.15-10ng/ml
Mouse TATC ELISA KIT <":////Mouse-Thrombin-antithrombin-complex-TATC-ELISA-Kit">
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