Mouse Thrombin-antithrombin co

Mouse Thrombin-antithrombin complex, TATC ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TATC,Thrombin-antithrombin complex, Thrombin-antithrombin,TAT, Thrombin, antithrombin

Search name

Mouse TATC ELISA KIT ,Mouse Thrombin-antithrombin complex ELISA KIT ,Mouse Thrombin-antithrombin ELISA KIT ,Mouse TAT ELISA KIT ,Mouse Thrombin ELISA KIT ,Mouse antithrombin ELISA KIT

Intended use

Mouse TATC ELISA KIT allows for the in vitro quantitative determination of mouse Thrombin-antithrombin complex concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse TATC ELISA KIT has been pre-coated with an antibody specific to Thrombin-antithrombin complex. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Thrombin-antithrombin complex and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Thrombin-antithrombin complex, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thrombin-antithrombin complex in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.15-10ng/ml

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