- ￥2980
- LSMBIO
- 78-5000pg/ml
- 46.79pg/ml
- 进口
- ELA-E1002m
- 2025年11月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测小鼠血清，血浆，组织匀浆等样本中目的蛋白的含量
- 适应物种：
小鼠
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
46.79pg/ml
- 规格：
96tests
Mouse 12-hydroxyeicosatetraenoic acid ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
12-Hydroxyeicosatetraenoic acid,12-HETE
Search name
Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit, Mouse 12-HETE ELISA Kit
Intended use
Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit allows for the in vitro quantitative determination of 12-hydroxyeicosatetraenoic acid,12-Hydroxyeicosatetraenoic
acid concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to 12-Hydroxyeicosatetraenoic acid, During the reaction, 12-Hydroxyeicosatetraenoic acid in the
sample or standard competes with a fixed amount of biotin-labeled 12-Hydroxyeicosatetraenoic acid for sites on a pre-coated Monoclonal
antibody specific to 12-Hydroxyeicosatetraenoic acid. Excess conjugate and unbound sample or standard are washed from the plate.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution
is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 12-Hydroxyeicosatetraenoic acid in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit Range 0.15-10ng/ml
Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit <":////Mouse-12-Hydroxyeicosattraenoic-acid-12-HETE-ELISA-Kit">
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