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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
兔
- 标记物:
Rabbit DNA repair protein RAD51 homolog 1,RAD51
- 样本:
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rabbit RAD51 ELISA KIT
Product Name:Rabbit RAD51 ELISA KIT
Packing:96T
Catalog No.:Rabbit RAD51 ELISA KIT
Gene Name:Rabbit RAD51
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Rabbit RAD51Rabbit RAD51
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rabbit RAD51 ELISA KITRabbit RAD51 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rabbit RAD51 ELISA KIT has been pre-coated with an Rabbit RAD51 antibody specific to Rabbit RAD51 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rabbit RAD51 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rabbit RAD51, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rabbit RAD51 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assay for Human Rad51-Mediated DNA Displacement Loop Formation
同源重组(Homologus Recombination) 是指发生在姐妹染色单体(sister chromatin) 之间或同一染色体上含有同源序列的DNA分子之间或分子之内的重新组合。同源重组需要一系列的蛋白质催化,如原核生物细胞内的RecA、RecBCD、RecF、RecO、RecR等;以及真核生物细胞内的Rad51、Mre11-Rad50等等。 在人类细胞中,同源重组由Rad51介导,在有ATP的条件下,Rad51聚合在单链DNA上,形成一个核蛋白
Biochemical Studies on Human Rad51-Mediated Homologous Recombination
Rad51-mediated pairing between homologous DNA sequences during homologous recombination (HR) plays pivotal roles in DNA double-strand break repair. The multi-step process occurs through cooperation of Rad51 and a number of accessory protein
In Vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
/Rad51 family of proteins are the key enzymes in this homology-directed repair pathway, as they perform DNA strand invasion and exchange, in concert with a host of ancillary factors. In vitro, the RecA/Rad51 family of proteins share similar enzymatic
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