- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-07698Ra
- 2025年07月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
兔
- 标记物：
Rabbit Aminopeptidase N,ANPEP
- 样本：
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rabbit ANPEP ELISA KIT
Product Name:Rabbit ANPEP ELISA KIT
Packing:96T
Catalog No.:Rabbit ANPEP ELISA KIT
Gene Name:Rabbit ANPEP
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Rabbit ANPEPRabbit ANPEP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rabbit ANPEP ELISA KITRabbit ANPEP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rabbit ANPEP ELISA KIT has been pre-coated with an Rabbit ANPEP antibody specific to Rabbit ANPEP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rabbit ANPEP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rabbit ANPEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rabbit ANPEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
一篇文献中的ELISA方法如下：Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
ELISA PROTOCOL 【Yale University 】
-nitrophenyl phosphate (5 mg/tablet). SigmaCat.# N93895) AP-conjugated secondary Ab: AP-Rabbit anti-mouse IgG(Zymed). Reconstitute in ddH2O as prescribed by manufacturer andaliquot and store at -70 °C.6) 100 mM EDTA pH
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