- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-05349Ra
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
兔
- 标记物：
Rabbit Actin,alpha skeletal muscle,ACTA1
- 样本：
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rabbit ACTA1 ELISA KIT
Product Name:Rabbit ACTA1 ELISA KIT
Packing:96T
Catalog No.:Rabbit ACTA1 ELISA KIT
Gene Name:Rabbit ACTA1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Rabbit ACTA1Rabbit ACTA1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rabbit ACTA1 ELISA KITRabbit ACTA1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rabbit ACTA1 ELISA KIT has been pre-coated with an Rabbit ACTA1 antibody specific to Rabbit ACTA1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rabbit ACTA1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rabbit ACTA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rabbit ACTA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Preparation of Conventional Actin from Skeletal Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866. 1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min. Use a big stir bar or overhead stirrer. Buffer G: 0.2 mM ATP, 0.5 mM DTT, 0.2 mM
Protein purification: skeletal muscle myosin
consists of an alpha-helical coiled-coil rod of 150 nm in length. The most important mechano-enzyme for muscle contraction. Material Source: rabbit skeletal muscle
Material 1. Source: rabbit skeletal muscle 2. Equipment: • mincer • glass-Teflon-homogenizer with pestle • sephacryl S-300 column (5 x 100 cm) • electrophoretic gels • centrifuge • sterile cheesecloth
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