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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
兔
- 标记物:
Rabbit nitric oxide synthase
- 样本:
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rabbit NOS ELISA Kit
Product Name:Rabbit NOS ELISA Kit
Packing:96T
Catalog No.:Rabbit NOS ELISA Kit
Gene Name:Rabbit NOS
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Rabbit NOSRabbit NOS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rabbit NOS ELISA KitRabbit NOS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rabbit NOS ELISA Kit has been pre-coated with an Rabbit NOS antibody specific to Rabbit NOS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rabbit NOS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rabbit NOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rabbit NOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Nitric oxide (NO) is an important bio-regulatory molecule in the nervous, immune and cardiovascular systems. The physiological involvement of NO in neuronal transmission, control of vascular tone, and immune response-induced cytostasis
Nitric Oxide Synthase Inhibitors
In 1990, several studies reported that an enhanced formation of endogenous nitric oxide (NO) contributes to the hypotension caused by endotoxin and tumor-necrosis factor-α (TNFα) (1 –3 ). In addition, it became apparent
Purification of the Inducible Nitric Oxide Synthase
Regulation of the inducible nitiric oxide synthase (iNOS) isozyme is distinct from the constitutively expressed endothelial (e) and neuronal (n) NOS isozymes in that it occurs at the level of gene expression and not by Ca+2 /calmodulin (CaM
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