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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
猪
- 标记物:
Porcine Matrix metalloproteinase 2,MMP2
- 样本:
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Porcine Matrix metalloproteinase 2, MMP2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MMP2,MMP-2,CLG4; CLG4A; MMP-II; MONA; TBE-1; 72 kDa gelatinase; 72 kDa type IV collagenase; collagenase type IV-A; gelatinase A; matrix metalloproteinase-2; matrix metalloproteinase-II; neutrophil gelatinase; matrix metallopeptidase 2 gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase
Search name
Porcine MMP2 ELISA KIT ,Porcine MMP-2 ELISA KIT ,Porcine CLG4 ELISA KIT ,Porcine CLG4A ELISA KIT ,Porcine MMP-II ELISA KIT ,Porcine MONA ELISA KIT ,Porcine TBE-1 ELISA KIT ,Porcine 72 kDa gelatinase ELISA KIT ,Porcine 72 kDa type IV collagenase ELISA KIT ,Porcine collagenase type IV-A ELISA KIT ,Porcine gelatinase A ELISA KIT ,Porcine matrix metalloproteinase-2 ELISA KIT ,Porcine matrix metalloproteinase-II ELISA KIT ,Porcine neutrophil gelatinase ELISA KIT ,Porcine matrix metallopeptidase 2 gelatinase A ELISA KIT ,Porcine 72kDa gelatinase ELISA KIT ,Porcine 72kDa type IV collagenase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine 72 kDa type IV collagenase concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to 72 kDa type IV collagenase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 72 kDa type IV collagenase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 72 kDa type IV collagenase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 72 kDa type IV collagenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Porcine-Matrix-metalloproteinase-2-MMP2-ELISA-Kit
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问:有人提到一种酶缓冲液提供酶反应条件,酶蛋白即催化底物明胶,不知道是怎么回事情。我要测定活性和分泌。我知道是要使用聚丙烯酰胺凝胶电泳的,请教具体的实验方法很所需要的试剂。谢谢! 答:Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. Type IV collagenase
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