- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-46050p
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
猪
- 标记物：
Porcine Niemann-Pick C1 protein,NPC1
- 样本：
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Porcine Niemann- Pick C1 protein, NPC1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NPC1,NPC; Niemann-Pick C1 protein; Niemann-Pick disease, type C1
Search name
Porcine NPC1 ELISA KIT ,Porcine NPC ELISA KIT ,Porcine Niemann-Pick C1 protein ELISA KIT ,Porcine Niemann-Pick disease type C1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine Niemann- Pick C1 protein, NPC1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NPC1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NPC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NPC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验亚单位(链)及分子量(kDa)：1条 激活产物：C1r� 生物学活性：丝氨酸蛋白酶，裂解C1s 补体成分：C1s 血清浓度(μg/ml)：50 分子量(kDa)：85 亚单位(链)及分子量(kDa)：1条 激活产物：C1s� 生物学活性：丝氨酸蛋白酶，裂解C4、C2 ELISA KIT
Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
Fastfilter Plasmid Maxi Kit Spin Protocol
as inoculun, streak it onto an agar plate containing the appropriate antibiotic for single colony isolation. Then pick a single colony and inoculate the 2-5ml starter culture as described above. Lyse bacterial cells with alkaline-SDS Solution:
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